Major Mastitis Pathogens Isolation

Selective Media - Triplate (blood agar/TKT agar/MacConkey agar)

Streptococcal Infections

Streptococcus agalactiae

Gram positive, small colonies catalase neg., esculine neg CAMP positive, hemolytic (beta)

Streptococcus species

Gram positive, small colonies catalase neg., esculine positive CAMP neg, non-hemolytic

Strep agalactiae - CAMP pos produces clearing (arrow-shape)

other Strep sp: esculine pos, CAMP negative